Application of precisioncut rat liver slice to study the. Engineered for standard biotherapeutic characterization analyses, the x500b qtof system is a true benchtop instrument, with streamlined processing to get to a consistent, accurate view of your biologic. Wat introduces a new data acquisition mode, sonar, for its xevo g2xs quadrupole timeofflight qtof mass spectrometer. Guidelines on specimen handling, cdc nih manual, 1984. Metabolites of glibenclamide detected using %a %b 0 98 e2 5. Data file naming is always qtof101, qtof102 etc where the 11 implies the year 2011 in this case. Comparison of pharmaceutical, illicit drug, alcohol, nicotine. Preparative hplc was carried out using shimadzu liquid.
Mass measurement the mass measurement accuracy of the instrume nt, in resolution mode, will be. Intended use of the xevo g2 xs qtof waters designed the orthogonal acceleration, timeofflight xevo g2 xs qtof for use as a research tool to deliver authenticated mass measurement. Thermo persuades me to q exactive focus orbitrap lcms. Column chromatography cc was performed using ods 4063. On xevo instruments, flip only the bottom toggle switch at the bottom in the middle of the back of the xevo. Mass spectrum of a therapeutic mab acquired on the sq detector 2. Waters introduces the new xevo tqd mass spectrometer, the. What is the part number for the tof turbo on a xevo g2s tof. Please contact us for complete waters refurbishedused mass spectrometer system, warranty and service contract details. Quantofs highfield pusher and dualstage reflectron, incorporating hightransmission parallel wire grids, reduce ion turnaround times due to prepush kinetic energy. Determination of mass resolution, sensitivity, and linear. Flip the electronics toggle switch the bottom switch of the two toggle switches on the back of the xevo down.
Waters xevo g2 vs agilent 6530 qtof chromatography forum. Tanshinones are active compounds that accumulate in the periderm, resulting in redcolored roots. Sep 14, 2011 watch this xevo g2 qtof animation to learn how you can obtain the highest quality, most comprehensive information that will enable you to make the right decisions quickly and confidently. Good laboratory practice, and consult your organization s safety representative for guidance. It shows how to import a workflow, and also briefly describes the investigate and report tabs. The waters acquity uplc iclass ensures fast analysis with high resolution power. Xevo g2 qtof and xevo tqs tandem ms waters corporation were used. This highperformance benchtop qtof gives you the confidence to make the right decisions quickly and easily using the highest quality, most comprehensive information.
Chromatographic separation was carried on a waters acquityuplc beh c 18 column 2. Waters designed the orthogonal accelera tion, timeofflight xevo g2s qtof for use as a research tool to deliver authenticated mass measurement. Stepwave ion optics provides unsurpassed levels of durable sensitivity. This manual is a companion to the masslynx nt user s guide supplied with the. Watch this xevo g2 qtof animation to learn how you can obtain the highest quality, most comprehensive information that will enable you to. Calibration fails on a qtof xevo or synapt due to poor. How to do an electronic reboot on a xevo series qtof. Sq detector 2 operators overview and maintenance guide support number. Ionbench with the waters xevo g2xs qtof mass spectrometer. An optional high mass quadrupole ion guide is available with a high mass cutoff of mz 100,000.
The tof ms experiments were performed by using a xevo g2 s q tof ms system waters corp. Symbol definition manufacturer authorized representative of the european community. Japan, photoluminescence and quantum yield were measured by fp6600 jasco corp. The extracts of reddish pigments and sappanwood were analyzed by both positive and negative mode and the results showed better sensitivity in the latter, so we chose the negative ion mode to get the data. Bch190ne58 detailed techniccal specifications noise enclosure for vacuum pumps noise reduction performance. Jun 15, 2017 esi q tof ms e conditions and data analysis. Ms parameters were optimized via a direct infusion of methamphetamine and thccooh 500 ngml in the initial mobile phase separately at 10. Comparison of different time of flightmass spectrometry. The xevo g2 s qtof is capable of quantifying small pharmaceutical compounds in biological matrices. A xevo g2s qtof mass spectrometer waters, milford, ma usa was used in positive esi mode for acquisition using mse, that allows both precursor and product ion data to be simultaneously acquired during a single run. This is not necessary if the pc is going to be rebooted in step 6. The waters xevo g2 qtof is coupled to a waters acquity uplc iclass, that offers the most powerful solutions to complex separation challenges.
How to shut down or vent a qtof or synapt for a planned. Xevo g2s qtof quadrupole timeofflight mass spectrometer. We are experiencing some noise problems with our new qtof mass spectrometer waters xevo g2 xs. Data were processed using either masslynx or unifi. Medical swab analysis using desorption electrospray. The xevo tqd joins waters xevo tq ms and xevo tq s as a member of waters growing family of xevo tandem quadrupole mass spectrometers, enabling our customers to consistently generate the highest quality quantitative lcmsms data across their organizations and make even better decisions with their data.
On the ms, flip the pump override switch to the right, to protect the vacuum assuming the unit is still pumped down. The test was performed on a waters acquity hclass uplcxevo g2s qtof system, with masslynx v4. Scientific analysis of tie luo, a qing dynasty calligraphy. The xevo g2xs qtof delivers not only conventional ms and msms methods of data acquisition, but also mse for comprehensive accurate mass precursor and fragment ion information from a single analysis, fastdda for rapid, intelligent automated accurate mass msms, and tofmrm for enhanced quantitative capability. Metabolite peaks were assigned to the accurate mass measurements using online metabolite databases as described in zhang et al. Ce instruments, if you are a bruker sales rep, change your nick, please. Analyses were performed using an external reference lockspraywithleucine. Power on the external epc if it is a xevo g2 all other instrument epcs will power on with the electronics wait 5 minutes for the epc to boot up. Considerations specific to the xevo g2 qtof solventleakage hazard the source exhaust system is designed to be robust and leaktight. In the ewre lab we have a watersmicromass xevo g2 xs qtof, quadrupole timeofflight mass spectrometer. Comprehensive characterization of lignans from forsythia.
So, the point is still centered on whether choose orbitrap or qtof. This is designed for use with both gas and liquid sample inlet device, specifically a dedicated uplc and api gc. Here, we performed metabolome and transcriptome analyses to investigate the changes of orangerooted. I am leaning towards water s xevo g2 xs qtof with uplc acquity hclass with pda or shimadzu qtof lcms9030. The waters xevo g2 q tof is coupled to a waters acquity uplc iclass, that offers the most powerful solutions to complex separation challenges. A arc 01 20005077e c pdf mass resolution resolution mode. How to reboot the epc on a xevo g2xs wkb56994 waters. Xevo g2xs tof is a benchtop timeofflight mass spectrometer designed. Xevo g2 xs tof performance, robustness, and flexibility made accessible to everyone. Q3, qtof or orbitrap lcms which will be better for. Overview to scale the picture below is a simulation at scale of the waters xevo g2 xs qtof lcms mass spectrometer system right side connected with an acquity uhplc left side, installed on a ionbench ms mass spec bench or table.
Now, i have narrowed the choices down to two models, thermo obitrap qe focus vs waters xevo g2 s qtof. The xevo g2s qtof is for research use only and is not intended for use in diagnostic applications. Check the vacuum view vacuum, then go to the vacuum tab make sure you have real vacuum readings not 1e8 for the tof vacuum. Metabolome and transcriptome analyses reveal quality change. Powered by quantof technology, xevo g2 tof delivers the most sensitive, exact mass, en quantitative and qualitative performance of any benchtop ms system. Mass spectrometry was performed on the xevo g2 q tof ms waters equipped with the electronic spray ion esi under negative ion mode. The extraction cone, sampling cone and capillary voltages were 4, 40 and 3000 v, respectively.
Lewis, paolo inglese, goncalo ds correia, trevor t. Which qtof is best abs triple tof5600 or waters g2s. Power off the external epc if it is a xevo g2 all other instrument epcs will power off with the electronics. Xevo g2 xs tof is a benchtop time of flight ms combining stepwave ion optics, xs collision cell and quantof technology for superior robustness, sensitivity and selectivity, resulting in high quality, comprehensive qualitative and quantitative information.
A waters acquity uplc system waters, milford, ma, usa was interfaced to a hybrid quadrupoleorthogonal acceleration tof mass spectrometer xevo g2 qtof, waters micromass, manchester, uk, using an orthogonal zsprayesi interface operating in both positive and negative ionisation modes. Jun 16, 2014 the waters xevo g2xs mass spectrometer, a new high performance benchtop quadrupole timeofflight qtof tm mass spectrometer, and the xevo tqs micro, a new and compact benchtop tandem. Xevo g2s tof is designed for scientists who need to identify and quantify the broadest range of compounds in complex and challenging samples. User s guide micromass uk limited floats road wythenshawe m23 9lz tel. For a xevo g2, poweroff the external epc all other instrument epcs poweroff with the electronics. Poweroff the ms breakers on the back starting with the epc, then electronics, then vacuum on synapt, qtof p or lct p instruments. Waters introduces xevo g2xs qtof and xevo tqs micro mass. Which one is the best lc msms system for both proteomics. For a single sample run you can use the following procedure or you can skip and use the worklist. Here, we demonstrate apcs with a xevo g2 s q tof from watersmicromass using an ion source originally designed for atmospheric pressureelectron capture dissociation apecd experiments. The exact mass of the newly synthesized compounds were measured by using a high resolution mass spectrometer xevo g2 s q tof.
The new q exactive has 140k resolution, an the cost is similar to a xevo g2 and agilent 6530 previous topic. Your quantitative and qualitative questions answered. High purity nitrogen n 2 was used as nebulizing gas, and ultrahigh pure helium he as collision gas. For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the xevo g2 xs tof provides maximum robustness with no compromise in performance, the highest quality, most comprehensive qualitative and quantitative information. Flip down the electronics and epc breaker switch to turn off the ms. Waters new xevo g2xs qtof mass spectrometer, bringing.
Uhplcqtof ms screening of pharmaceuticals and their. Waters acquity hclass uplc with pda and column manager. Medical swab analysis using desorption electrospray ionization mass spectrometry desims a novel noninvasive approach for mucosal diagnostics pamela pruski, david a. However, lines with orange roots have been observed in cultivated fields. In detail, the instrument is connected to a waters. Hemostatic chemical constituents from natural medicine. Uplc xevo g2 s q tof and separated with an hss t3 column as described by zhang et al. The xevo g2 xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tof mrm, stepwave tm and quantof tm to deliver optimum levels of robust. Waters advances proteomics and lipidomics research with novel. The lc front end employed was either the acquity iclass or acquity mclass with the ionkey ms source. Anyone with real experience on either obitrap qe or xevo g2 s qtof, please, give me your suggestions, especially information for performance and stability, calibriation frequency, etc.
Tof g2 s ms install 682,00 1 682,00 18 176001994 ms ref stds. May 2012 720004348en ao pdf ms sensitivity esi the peak at mz 503 from a solution of 500 pgl raffinose in 7030 acetonitrilewater no additives, will have an intensity of greater than 7,200 counts per second. View online or download waters xevo g2 xs qtof overview and maintenance manual. Xevo g2 qtof, a quadrupole timeofflight mass spectrometer, delivers everything you need for quantitative and qualitative analysis.
Waters unifixevog2 s my work xevo g2 s xevo g2 s qtof manual tuning console navigation system xevo g2 s xevo g2 s qtof menu summary setup maintain tools setup configure. The xevo g2 xs qtof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comprehensive qualitative and quantitative information. Metabolomics analysis reveals the metabolic and functional. Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% lc eluate. Excellent resolution, sensitivity of 3 to 24 pgml, and excellent linear dynamic range of 3. Compared to the xevo tq s, already the most sensitive qqq instrument on the market, the. View and download waters xevo g2 qtof operator s, overview and maintenance manual online. The quadrupole ion guide has a high mass cutoff of mz 16,000. Calibration or calibration check fails on a xevo or synapt qtof calibration runs but some peaks do not match and cannot be manually added only one peak may be matched peaks may be flagged with exclamation marks.
The analysis of the dichloromethane subfractions was performed on a waters xevo g2 q tof mass spectrometer waters ms technologies, manchester, uk, which was equipped with an electrospray ionization interface with waters acquity uhplc system waters, co. Qtof premier operators guide 71500089402 revision b vtp up. Electrospray introduction 43 postcolumn splitting 45. Uplcqtof msbased metabolomics and qrtpcr in enzyme gene. Surface functionalization of silica by sih activation of. Xevo g2 s qt of xevo tq s performance, robustness, and flexibility made accessible to everyone for the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples.
Xevo g2 xs qtof the xevo g2 xs qtof combines stepwave ion optics, xs. If you want the fan separately, order part number 700012677. High resolution mass spectrometry quantitation explained. A waters xevo g2 xs q tof quadrupole timeofflight mass spectrometer usa hyphenated with the waters acquity uplc equipped with an esi source was applied for rapidly identifying the major constituents in ethyl acetate fraction showing hemostatic activity, based on systematic database retrieval. The xevo g2 s q tof mass spectrometer was run in negative mode.
Qtof 2 users guide micromass uk limited floats road. The xevo g2xs qtof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comp rehensive qualitative and quantitative information. Method of atmospheric pressure charge stripping for. The data were collected for each test sample from 50 da to 1500 da. Whether youre a scientist who wants to truly understand your samples, or a lab manager with urgent deadlines to meet, the xevo g2 xs qtof is the ultimate solution.
Nitrogen used as cone and desolvation gas were set to 30 and 800lh, respectively. Considerations specific to the xevo g2 s qtof solventleakage hazard the source exhaust system is designed to be robust and leaktight. Mass spectra were conducted on a waters xevo g2 s q tof lcms spectrometer. Tof rf settings positive ion mode collision cell system 1 6. The xevo g2 xs qtof mass spectrometer combines the new xs collision cell with the proven, signature technologies of tof mrm, stepwavetm and quantoftm to deliver optimum levels of robust sensitivity and selectivity, resulting in the detection of more lowlevel compounds than ever before. The xevo g2 xs qtof is for research use only and is not intended for use in diagnostic applications. Waters xevo g2xs qtof overview and maintenance manual pdf.
Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% lc. This kit is designed for use by a waters certified engineer or user as part of a performance maintenance or qualification activity. The instrument is developed to give the most accurate and reproducible separation of complex sample matrices. Xevo g2 qtof laboratory equipment pdf manual download. Full scan and tof mrm mode of data acquisition were used. The ftir spectra were recorded on an ftir spectrometer ftir4100, jasco corp. View online or download waters xevo g2xs qtof overview and maintenance manual. The xevo g2s qtofms with ms e mode was adopted to give high resolution mass of characteristic components of dyestuff. The tof g2 s sample kit 1 is intended for the verification of system performance for tof g2 s.
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